欢迎访问成都乐美天医药科技有限公司

World Journal of Microbiology and Biotechnology-20 June 2018

发布时间:2022-09-19
作者:未知
阅读次数:93
文章字数:1297

期刊名:World Journal of Microbiology and Biotechnology
文献编号:DOI 10.1007/s11274-018-2464-1
文献地址:https://link.springer.com/article/10.1007%2Fs13318-017-0444-8
发表日期:

Abstract

The present study has focused on the effects of hypericin (Hyp) based photodynamic inactivation (PDI) of Escherichia coli (E. coli). To evaluate the efficiency of Hyp based PDI of E. coli, single factor experiments and response surface optimization experiment were conducted to obtain the optimum parameter values (36 µM Hyp, 5.9 J cm−2 light dose: 16.4 mW cm−2, 60 W, 260 s, 590 nm and 68 min incubation time) and finally achieved a 4.1 log CFU mL−1 decrease of E. coli. Cell-Hyp interaction and intracellular reactive oxygen species (ROS) level were detected by fluorescence spectrometric photometer. Data indicated that Hyp possessed a strong ability to bind with cells. In addition, a significant increase was observed in intracellular ROS level after Hyp-based photosensitization treatment. Therefore, Hyp-based photosensitization seems to be a promising method to efficiently inactivate E. coli. It is expected to be a safe, efficient, low cost and practical method which can be applied in the field of food safety.


Hypericin used as a photosensitizer in present study was obtained from Chengdu Desite Biological Technology Co., Ltd. (Chengdu, China).

相关文章 产品推荐
QQ咨询
售前咨询:
售前咨询:
代理合作:
集采合作: 点击这里给我发消息